Detailed information of TA system TA111552

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64207..64471
• Replicon: plasmid unnamed2
• Accession: NZ_CP032264
• Organism: Escherichia coli strain AR_0089

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: AM468_RS01370
• Protein ID: WP_001303307.1
• Coordinates: 64207..64359 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	64409..64471 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM468_RS01335	59490..60152	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
AM468_RS01340	60224..60433	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AM468_RS27080	60825..61001	+	177	WP_001054900.1	hypothetical protein	-
-	61487..61538	+	52	NuclAT_1	-	-
-	61487..61538	+	52	NuclAT_1	-	-
-	61487..61538	+	52	NuclAT_1	-	-
-	61487..61538	+	52	NuclAT_1	-	-
AM468_RS01355	61722..62744	+	1023	WP_001572805.1	IS21-like element IS100 family transposase	-
AM468_RS01360	62741..63523	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
AM468_RS01365	63884..64135	+	252	WP_001291965.1	hypothetical protein	-
AM468_RS01370	64207..64359	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	64409..64471	+	63	NuclAT_0	-	Antitoxin
-	64409..64471	+	63	NuclAT_0	-	Antitoxin
-	64409..64471	+	63	NuclAT_0	-	Antitoxin
-	64409..64471	+	63	NuclAT_0	-	Antitoxin
AM468_RS01375	64651..65859	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
AM468_RS01380	65878..66948	+	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
AM468_RS01385	66941..69232	+	2292	WP_001289271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..101614	101614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T111552 WP_001303307.1 NZ_CP032264:c64359-64207 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T111552 NZ_CP032264:c64359-64207 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT111552 NZ_CP032264:64409-64471 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin