Detailed information of TA system TA111542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42548..42802
• Replicon: plasmid unnamed1
• Accession: NZ_CP032263
• Organism: Escherichia coli strain AR_0089

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM468_RS00270
• Protein ID: WP_001351576.1
• Coordinates: 42596..42802 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42548..42609 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM468_RS00245	39296..40042	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
AM468_RS00250	40101..40961	+	861	WP_000704513.1	alpha/beta hydrolase	-
AM468_RS00255	41064..41624	+	561	WP_000139315.1	fertility inhibition protein FinO	-
AM468_RS00260	41760..41972	+	213	WP_001299730.1	ANR family transcriptional regulator	-
AM468_RS27335	42218..42292	+	75	Protein_49	endonuclease	-
-	42548..42609	-	62	NuclAT_1	-	Antitoxin
-	42548..42609	-	62	NuclAT_1	-	Antitoxin
-	42548..42609	-	62	NuclAT_1	-	Antitoxin
-	42548..42609	-	62	NuclAT_1	-	Antitoxin
AM468_RS00270	42596..42802	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM468_RS00275	43086..43343	+	258	WP_000083845.1	replication regulatory protein RepA	-
AM468_RS00285	43575..43649	+	75	WP_001442103.1	RepA leader peptide Tap	-
AM468_RS00290	43642..44124	+	483	WP_001273588.1	hypothetical protein	-
AM468_RS00295	44117..44974	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
AM468_RS00305	45937..46188	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AM468_RS00310	46185..46472	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / iroN / iroE / iroD / iroC / iroB	1..155784	155784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T111542 WP_001351576.1 NZ_CP032263:42596-42802 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T111542 NZ_CP032263:42596-42802 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT111542 NZ_CP032263:c42609-42548 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin