Detailed information of TA system TA111537

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1669..1939
• Replicon: plasmid unnamed1
• Accession: NZ_CP032263
• Organism: Escherichia coli strain AR_0089

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM468_RS00020
• Protein ID: WP_001312861.1
• Coordinates: 1781..1939 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1669..1732 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM468_RS00010	351..785	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
AM468_RS00015	782..1501	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
AM468_RS27070	1513..1701	-	189	WP_001299721.1	hypothetical protein	-
-	1513..1737	+	225	NuclAT_0	-	-
-	1513..1737	+	225	NuclAT_0	-	-
-	1513..1737	+	225	NuclAT_0	-	-
-	1513..1737	+	225	NuclAT_0	-	-
-	1669..1732	-	64	-	-	Antitoxin
AM468_RS00020	1781..1939	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM468_RS27330	2630..2836	+	207	WP_000547939.1	hypothetical protein	-
AM468_RS00040	2861..3148	+	288	WP_000107535.1	hypothetical protein	-
AM468_RS00045	3269..4090	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AM468_RS00050	4387..4989	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
AM468_RS00055	5310..5693	+	384	WP_001151524.1	relaxosome protein TraM	-
AM468_RS00060	5880..6569	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / iroN / iroE / iroD / iroC / iroB	1..155784	155784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T111537 WP_001312861.1 NZ_CP032263:1781-1939 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T111537 NZ_CP032263:1781-1939 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT111537 NZ_CP032263:c1732-1669 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin