Detailed information of TA system TA111533

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3830..4252
• Replicon: plasmid unnamed4
• Accession: NZ_CP032262
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: AM446_RS27945
• Protein ID: WP_001302184.1
• Coordinates: 4094..4252 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	3830..4029 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS27925	363..596	+	234	WP_000006018.1	DUF905 domain-containing protein	-
AM446_RS27930	655..2613	+	1959	WP_001145501.1	ParB/RepB/Spo0J family partition protein	-
AM446_RS27935	2668..3102	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
AM446_RS27940	3099..3818	+	720	WP_001276230.1	plasmid SOS inhibition protein A	-
-	3830..4029	+	200	NuclAT_0	-	Antitoxin
-	3830..4029	+	200	NuclAT_0	-	Antitoxin
-	3830..4029	+	200	NuclAT_0	-	Antitoxin
-	3830..4029	+	200	NuclAT_0	-	Antitoxin
AM446_RS27945	4094..4252	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM446_RS27965	5168..5455	+	288	WP_000107526.1	hypothetical protein	-
AM446_RS27970	5574..6395	+	822	WP_162897670.1	DUF945 domain-containing protein	-
AM446_RS27975	6691..7293	-	603	Protein_8	transglycosylase SLT domain-containing protein	-
AM446_RS27980	7614..7997	+	384	WP_001151538.1	relaxosome protein TraM	-
AM446_RS27985	8184..8873	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..75026	75026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T111533 WP_001302184.1 NZ_CP032262:4094-4252 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T111533 NZ_CP032262:4094-4252 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT111533 NZ_CP032262:3830-4029 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin