Detailed information of TA system TA111526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3929439..3930133
• Replicon: chromosome
• Accession: NZ_CP032261
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: AM446_RS22035
• Protein ID: WP_001521903.1
• Coordinates: 3929439..3929837 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM446_RS22040
• Protein ID: WP_000554758.1
• Coordinates: 3929840..3930133 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS22005	3924609..3925853	-	1245	WP_000189541.1	esterase FrsA	-
-	3925269..3925349	-	81	NuclAT_11	-	-
-	3925269..3925349	-	81	NuclAT_11	-	-
-	3925269..3925349	-	81	NuclAT_11	-	-
-	3925269..3925349	-	81	NuclAT_11	-	-
AM446_RS22010	3925945..3926403	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AM446_RS22015	3926664..3928121	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AM446_RS22020	3928178..3928530	-	353	Protein_3739	peptide chain release factor H	-
AM446_RS22025	3928526..3928732	-	207	Protein_3740	RtcB family protein	-
AM446_RS22030	3928977..3929429	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
AM446_RS22035	3929439..3929837	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM446_RS22040	3929840..3930133	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM446_RS22045	3930185..3931240	-	1056	WP_001226177.1	DNA polymerase IV	-
AM446_RS22050	3931311..3932096	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AM446_RS22055	3932068..3933780	+	1713	Protein_3746	flagellar biosynthesis protein FlhA	-
AM446_RS22060	3933859..3934212	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
AM446_RS22065	3934269..3935018	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3919376..3930133	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T111526 WP_001521903.1 NZ_CP032261:c3929837-3929439 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T111526 NZ_CP032261:c3929837-3929439 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT111526 WP_000554758.1 NZ_CP032261:c3930133-3929840 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT111526 NZ_CP032261:c3930133-3929840 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3