Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3060634..3061136 Replicon chromosome
Accession NZ_CP032261
Organism Escherichia coli strain AR_0067

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM446_RS17675 Protein ID WP_000767829.1
Coordinates 3060634..3060888 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM446_RS17680 Protein ID WP_001259255.1
Coordinates 3060885..3061136 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM446_RS17645 3056416..3057774 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM446_RS17650 3057764..3057826 - 63 WP_010723108.1 membrane protein YoeI -
AM446_RS17655 3058033..3058491 - 459 WP_000526135.1 IS200/IS605 family transposase -
AM446_RS17665 3058752..3059681 - 930 WP_023154044.1 LysR family transcriptional regulator -
AM446_RS17670 3059727..3060551 - 825 WP_000754758.1 SDR family oxidoreductase -
AM446_RS17675 3060634..3060888 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM446_RS17680 3060885..3061136 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM446_RS17685 3061419..3061469 + 51 WP_001364200.1 his operon leader peptide -
AM446_RS17695 3061615..3062514 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
AM446_RS17700 3062520..3063824 + 1305 WP_021523144.1 histidinol dehydrogenase -
AM446_RS17705 3063821..3064891 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AM446_RS17710 3064891..3065958 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3058033..3058491 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T111522 WP_000767829.1 NZ_CP032261:c3060888-3060634 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111522 NZ_CP032261:c3060888-3060634 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111522 WP_001259255.1 NZ_CP032261:c3061136-3060885 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111522 NZ_CP032261:c3061136-3060885 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References