111522

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3060634..3061136 Replicon chromosome
Accession NZ_CP032261
Organism Escherichia coli strain AR_0067

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM446_RS17675 Protein ID WP_000767829.1
Coordinates 3060634..3060888 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM446_RS17680 Protein ID WP_001259255.1
Coordinates 3060885..3061136 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM446_RS17645 3056416..3057774 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM446_RS17650 3057764..3057826 - 63 WP_010723108.1 membrane protein YoeI -
AM446_RS17655 3058033..3058491 - 459 WP_000526135.1 IS200/IS605 family transposase -
AM446_RS17665 3058752..3059681 - 930 WP_023154044.1 LysR family transcriptional regulator -
AM446_RS17670 3059727..3060551 - 825 WP_000754758.1 SDR family oxidoreductase -
AM446_RS17675 3060634..3060888 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM446_RS17680 3060885..3061136 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM446_RS17685 3061419..3061469 + 51 WP_001364200.1 his operon leader peptide -
AM446_RS17695 3061615..3062514 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
AM446_RS17700 3062520..3063824 + 1305 WP_021523144.1 histidinol dehydrogenase -
AM446_RS17705 3063821..3064891 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AM446_RS17710 3064891..3065958 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3058033..3058491 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T111522 WP_000767829.1 NZ_CP032261:c3060888-3060634 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111522 NZ_CP032261:c3060888-3060634 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111522 WP_001259255.1 NZ_CP032261:c3061136-3060885 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111522 NZ_CP032261:c3061136-3060885 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References