Detailed information of TA system TA111508

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2250228..2250599
• Replicon: chromosome
• Accession: NZ_CP032261
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: AM446_RS13245
• Protein ID: WP_001443846.1
• Coordinates: 2250450..2250599 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2250228..2250406 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS13220	2246183..2247556	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
AM446_RS13225	2247685..2248620	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AM446_RS13230	2248672..2249907	-	1236	WP_000040839.1	site-specific integrase	-
AM446_RS13235	2249909..2250124	-	216	WP_000079604.1	excisionase XisR	-
AM446_RS13240	2250224..2250412	-	189	WP_001302840.1	DUF1187 family protein	-
-	2250228..2250406	+	179	NuclAT_0	-	Antitoxin
-	2250228..2250406	+	179	NuclAT_0	-	Antitoxin
-	2250228..2250406	+	179	NuclAT_0	-	Antitoxin
-	2250228..2250406	+	179	NuclAT_0	-	Antitoxin
AM446_RS13245	2250450..2250599	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AM446_RS13250	2250655..2251464	-	810	WP_000166313.1	recombination protein RecT	-
AM446_RS13255	2251457..2254057	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
AM446_RS13260	2254159..2254434	-	276	WP_001344816.1	hypothetical protein	-
AM446_RS13265	2254509..2254679	-	171	WP_001352098.1	conserved protein, Rac prophage	-
AM446_RS13270	2254679..2254900	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2248672..2299810	51138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T111508 WP_001443846.1 NZ_CP032261:c2250599-2250450 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T111508 NZ_CP032261:c2250599-2250450 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT111508 NZ_CP032261:2250228-2250406 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin