Detailed information of TA system TA111497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 20788..21434
• Replicon: plasmid unnamed2
• Accession: NZ_CP032259
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM446_RS00985
• Protein ID: WP_000269912.1
• Coordinates: 20788..21135 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM446_RS00990
• Protein ID: WP_001259435.1
• Coordinates: 21135..21434 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS00960	16734..17351	+	618	WP_001523080.1	tail fiber assembly protein	-
AM446_RS00965	17315..17860	-	546	WP_001704996.1	transferase	-
AM446_RS00970	18489..19325	+	837	WP_001139597.1	hypothetical protein	-
AM446_RS00975	19969..20256	+	288	WP_000356589.1	hypothetical protein	-
AM446_RS00980	20280..20543	+	264	WP_000424604.1	hypothetical protein	-
AM446_RS00985	20788..21135	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM446_RS00990	21135..21434	+	300	WP_001259435.1	XRE family transcriptional regulator	Antitoxin
AM446_RS00995	21598..21978	+	381	WP_001523084.1	hypothetical protein	-
AM446_RS01000	22082..22507	-	426	WP_016240784.1	hypothetical protein	-
AM446_RS01005	22609..22869	+	261	WP_001523087.1	helix-turn-helix domain-containing protein	-
AM446_RS01010	23492..23680	+	189	WP_033545979.1	hypothetical protein	-
AM446_RS01015	23930..25102	+	1173	WP_000952372.1	IS21-like element IS21 family transposase	-
AM446_RS01020	25102..25899	+	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49921	49921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T111497 WP_000269912.1 NZ_CP032259:20788-21135 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T111497 NZ_CP032259:20788-21135 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11046.87 Da
• Isoelectric Point: 5.2090

>AT111497 WP_001259435.1 NZ_CP032259:21135-21434 [Escherichia coli]
MRTLDEAIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT111497 NZ_CP032259:21135-21434 [Escherichia coli]
ATGAGAACATTAGATGAGGCGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA