Detailed information of TA system TA111493

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90592..90862
• Replicon: plasmid unnamed1
• Accession: NZ_CP032258
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM446_RS00645
• Protein ID: WP_001312861.1
• Coordinates: 90704..90862 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90592..90654 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS00620	86360..86899	+	540	WP_000290841.1	single-stranded DNA-binding protein	-
AM446_RS00625	86962..87195	+	234	WP_000005990.1	DUF905 family protein	-
AM446_RS00630	87260..89218	+	1959	WP_162897652.1	ParB/RepB/Spo0J family partition protein	-
AM446_RS00635	89273..89707	+	435	WP_119163883.1	conjugation system SOS inhibitor PsiB	-
AM446_RS00640	89704..90423	+	720	WP_001526045.1	plasmid SOS inhibition protein A	-
-	90435..90660	+	226	NuclAT_0	-	-
-	90435..90660	+	226	NuclAT_0	-	-
-	90435..90660	+	226	NuclAT_0	-	-
-	90435..90660	+	226	NuclAT_0	-	-
-	90592..90654	-	63	-	-	Antitoxin
AM446_RS00645	90704..90862	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM446_RS28780	91516..91722	+	207	WP_000275859.1	hypothetical protein	-
AM446_RS00665	91747..92034	+	288	WP_000107535.1	hypothetical protein	-
AM446_RS00670	92154..92975	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AM446_RS00675	93272..93874	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM446_RS00680	94205..94588	+	384	WP_001151566.1	relaxosome protein TraM	-
AM446_RS00685	94722..95399	+	678	WP_001348626.1	PAS domain-containing protein	-
AM446_RS00690	95487..95714	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..128033	128033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T111493 WP_001312861.1 NZ_CP032258:90704-90862 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T111493 NZ_CP032258:90704-90862 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT111493 NZ_CP032258:c90654-90592 [Escherichia coli]
ACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin