Detailed information of TA system TA111487

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1851..2090
• Replicon: plasmid unnamed1
• Accession: NZ_CP032258
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: AM446_RS00030
• Protein ID: WP_023144756.1
• Coordinates: 1956..2090 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1851..1911 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS00010	182..742	+	561	WP_000139341.1	fertility inhibition protein FinO	-
AM446_RS00015	873..1085	+	213	WP_013023861.1	hypothetical protein	-
AM446_RS00025	1598..1884	+	287	Protein_3	DUF2726 domain-containing protein	-
-	1851..1911	-	61	NuclAT_1	-	Antitoxin
-	1851..1911	-	61	NuclAT_1	-	Antitoxin
-	1851..1911	-	61	NuclAT_1	-	Antitoxin
-	1851..1911	-	61	NuclAT_1	-	Antitoxin
AM446_RS00030	1956..2090	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM446_RS00035	2387..2641	+	255	WP_000083850.1	replication regulatory protein RepA	-
AM446_RS28760	2747..2881	+	135	Protein_6	protein CopA/IncA	-
AM446_RS00045	2878..2952	+	75	WP_031943482.1	RepA leader peptide Tap	-
AM446_RS00050	2945..3802	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
AM446_RS28435	4504..4644	+	141	WP_001333237.1	hypothetical protein	-
AM446_RS00060	4742..5395	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
AM446_RS28440	5488..5745	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
AM446_RS00065	5678..6079	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..128033	128033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T111487 WP_023144756.1 NZ_CP032258:1956-2090 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T111487 NZ_CP032258:1956-2090 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT111487 NZ_CP032258:c1911-1851 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin