Detailed information of TA system TA111482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2146159..2146754
• Replicon: chromosome
• Accession: NZ_CP032257
• Organism: Pseudomonas aeruginosa strain AR_0111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: AM490_RS11065
• Protein ID: WP_003113526.1
• Coordinates: 2146476..2146754 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM490_RS11060
• Protein ID: WP_003113527.1
• Coordinates: 2146159..2146464 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM490_RS11040	2141866..2142657	+	792	WP_034019065.1	IS21-like element helper ATPase IstB	-
AM490_RS11045	2142864..2143727	-	864	WP_034019067.1	integrase domain-containing protein	-
AM490_RS11050	2144330..2145487	-	1158	WP_034019070.1	hypothetical protein	-
AM490_RS11060	2146159..2146464	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
AM490_RS11065	2146476..2146754	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM490_RS11075	2147083..2149311	+	2229	WP_033942306.1	TonB-dependent receptor	-
AM490_RS11080	2149381..2150028	-	648	WP_003095021.1	carbonate dehydratase	-
AM490_RS11085	2150090..2151328	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T111482 WP_003113526.1 NZ_CP032257:c2146754-2146476 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T111482 NZ_CP032257:c2146754-2146476 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT111482 WP_003113527.1 NZ_CP032257:c2146464-2146159 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT111482 NZ_CP032257:c2146464-2146159 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3