Detailed information of TA system TA111471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 122673..123400
• Replicon: plasmid unnamed1
• Accession: NZ_CP032241
• Organism: Klebsiella pneumoniae strain XJ-K2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: D3N36_RS27470
• Protein ID: WP_011251285.1
• Coordinates: 122673..122984 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3N36_RS27475
• Protein ID: WP_011251286.1
• Coordinates: 122981..123400 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3N36_RS27430	117684..118194	+	511	Protein_133	hypothetical protein	-
D3N36_RS27435	118184..118624	+	441	WP_011251275.1	hypothetical protein	-
D3N36_RS27440	119049..120005	-	957	WP_011251280.1	DsbA family protein	-
D3N36_RS27445	120065..120406	-	342	WP_011251281.1	hypothetical protein	-
D3N36_RS27450	120420..120731	-	312	WP_011251282.1	hypothetical protein	-
D3N36_RS27455	120748..121182	-	435	Protein_138	thioredoxin fold domain-containing protein	-
D3N36_RS27460	121199..121896	-	698	Protein_139	IS1 family transposase	-
D3N36_RS27465	122010..122468	+	459	Protein_140	DDE-type integrase/transposase/recombinase	-
D3N36_RS27470	122673..122984	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
D3N36_RS27475	122981..123400	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
D3N36_RS27480	123547..124514	+	968	Protein_143	IS5 family transposase	-
D3N36_RS27485	124616..124951	-	336	Protein_144	conjugal transfer protein TraI	-
D3N36_RS27490	124965..125753	-	789	WP_040217257.1	hypothetical protein	-
D3N36_RS27495	125774..126217	-	444	WP_048333569.1	hypothetical protein	-
D3N36_RS29640	126813..127568	+	756	Protein_147	hypothetical protein	-
D3N36_RS27505	127761..128231	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..219775	219775
	-	inside	IScluster/Tn	-	-	115764..140069	24305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T111471 WP_011251285.1 NZ_CP032241:122673-122984 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T111471 NZ_CP032241:122673-122984 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT111471 WP_011251286.1 NZ_CP032241:122981-123400 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT111471 NZ_CP032241:122981-123400 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1