Detailed information of TA system TA111447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13809..14466
• Replicon: plasmid pTB221
• Accession: NZ_CP032238
• Organism: Escherichia coli strain ECCWS199

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: D3W53_RS24225
• Protein ID: WP_000270043.1
• Coordinates: 14116..14466 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3W53_RS24220
• Protein ID: WP_000124640.1
• Coordinates: 13809..14111 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W53_RS24170	9434..9862	+	429	WP_000591074.1	hypothetical protein	-
D3W53_RS24175	9919..10278	+	360	WP_000422768.1	hypothetical protein	-
D3W53_RS24180	10278..10724	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
D3W53_RS24185	10721..11239	+	519	WP_000210756.1	nitrite reductase	-
D3W53_RS24190	11239..11469	+	231	WP_000972663.1	hypothetical protein	-
D3W53_RS24195	11456..12313	+	858	WP_001167032.1	hypothetical protein	-
D3W53_RS24200	12339..12527	+	189	WP_001270411.1	hypothetical protein	-
D3W53_RS24205	12544..13071	+	528	WP_001236377.1	thermonuclease family protein	-
D3W53_RS24210	13129..13401	+	273	WP_001043047.1	HU family DNA-binding protein	-
D3W53_RS24215	13489..13782	+	294	WP_001239997.1	chromosome segregation protein ParM	-
D3W53_RS24220	13809..14111	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
D3W53_RS24225	14116..14466	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3W53_RS24230	14629..15177	+	549	WP_001061574.1	transcriptional regulator	-
D3W53_RS24240	15518..15712	+	195	WP_000343597.1	hypothetical protein	-
D3W53_RS24245	15723..16094	+	372	WP_000516916.1	hypothetical protein	-
D3W53_RS24250	16087..16395	+	309	Protein_27	hypothetical protein	-
D3W53_RS24255	16565..17764	-	1200	WP_000948429.1	IS91 family transposase	-
D3W53_RS24260	17774..17962	-	189	WP_000957857.1	hypothetical protein	-
D3W53_RS24270	18260..18595	-	336	WP_000683476.1	hypothetical protein	-
D3W53_RS24275	18692..19180	+	489	WP_001273096.1	DUF1643 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / qnrVC4 / blaOXA-10 / ant(3'')-Ia / dfrA14 / mph(A)	-	1..162163	162163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T111447 WP_000270043.1 NZ_CP032238:c14466-14116 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T111447 NZ_CP032238:c14466-14116 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT111447 WP_000124640.1 NZ_CP032238:c14111-13809 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT111447 NZ_CP032238:c14111-13809 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9