111446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4266561..4267058 Replicon chromosome
Accession NZ_CP032237
Organism Escherichia coli strain ECCWS199

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D3W53_RS21865 Protein ID WP_001173343.1
Coordinates 4266561..4266734 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag D3W53_RS21870 Protein ID WP_071597404.1
Coordinates 4266762..4267058 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W53_RS21855 4263226..4264683 + 1458 WP_072778207.1 dipeptide/tripeptide permease DtpC -
D3W53_RS21860 4264920..4266437 + 1518 WP_001295074.1 lysine--tRNA ligase -
D3W53_RS21865 4266561..4266734 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D3W53_RS21870 4266762..4267058 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D3W53_RS21875 4267285..4267557 - 273 WP_000405647.1 N-acetyltransferase -
D3W53_RS21880 4267569..4267799 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
D3W53_RS21885 4267980..4269611 + 1632 WP_001565376.1 sensor histidine kinase -
D3W53_RS21890 4269608..4270327 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4257703..4267058 9355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T111446 WP_001173343.1 NZ_CP032237:c4266734-4266561 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T111446 NZ_CP032237:c4266734-4266561 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT111446 WP_071597404.1 NZ_CP032237:c4267058-4266762 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT111446 NZ_CP032237:c4267058-4266762 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References