Detailed information of TA system TA111444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3783568..3784262
• Replicon: chromosome
• Accession: NZ_CP032237
• Organism: Escherichia coli strain ECCWS199

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: D3W53_RS19445
• Protein ID: WP_001263493.1
• Coordinates: 3783568..3783966 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: D3W53_RS19450
• Protein ID: WP_000554757.1
• Coordinates: 3783969..3784262 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W53_RS19415	3778568..3779812	-	1245	WP_000189541.1	esterase FrsA	-
-	3779228..3779308	-	81	NuclAT_11	-	-
-	3779228..3779308	-	81	NuclAT_11	-	-
-	3779228..3779308	-	81	NuclAT_11	-	-
-	3779228..3779308	-	81	NuclAT_11	-	-
D3W53_RS19420	3779904..3780362	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D3W53_RS19425	3780623..3782080	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
D3W53_RS19430	3782137..3782658	-	522	Protein_3588	peptide chain release factor H	-
D3W53_RS19435	3782657..3782860	-	204	Protein_3589	RNA ligase RtcB family protein	-
D3W53_RS19440	3783106..3783558	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
D3W53_RS19445	3783568..3783966	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D3W53_RS19450	3783969..3784262	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D3W53_RS19455	3784314..3785369	-	1056	WP_001226164.1	DNA polymerase IV	-
D3W53_RS19460	3785440..3786225	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
D3W53_RS19465	3786197..3787909	+	1713	Protein_3595	flagellar biosynthesis protein FlhA	-
D3W53_RS19470	3788133..3788629	-	497	Protein_3596	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T111444 WP_001263493.1 NZ_CP032237:c3783966-3783568 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T111444 NZ_CP032237:c3783966-3783568 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT111444 WP_000554757.1 NZ_CP032237:c3784262-3783969 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT111444 NZ_CP032237:c3784262-3783969 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6