Detailed information of TA system TA111428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 62539..63204
• Replicon: plasmid pYR4
• Accession: NZ_CP032236
• Organism: Yersinia ruckeri strain NHV_3758

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: CLC90_RS17520
• Protein ID: WP_038251855.1
• Coordinates: 62881..63204 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CLC90_RS17515
• Protein ID: WP_004720859.1
• Coordinates: 62539..62835 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLC90_RS17495	57836..59761	-	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-
CLC90_RS17500	59763..60107	-	345	WP_004720848.1	hypothetical protein	-
CLC90_RS17505	60376..60699	+	324	WP_004720850.1	hypothetical protein	-
CLC90_RS17510	61238..62051	-	814	Protein_60	DUF945 domain-containing protein	-
CLC90_RS17515	62539..62835	-	297	WP_004720859.1	helix-turn-helix domain-containing protein	Antitoxin
CLC90_RS17520	62881..63204	-	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLC90_RS17525	63356..63589	+	234	WP_038251853.1	hypothetical protein	-
CLC90_RS17530	63597..64094	-	498	WP_126940174.1	hypothetical protein	-
CLC90_RS17535	64091..64450	-	360	WP_126940175.1	DUF3085 domain-containing protein	-
CLC90_RS17540	65209..65919	-	711	WP_126940176.1	type I restriction-modification system subunit M	-
CLC90_RS17545	66065..66211	-	147	WP_126940177.1	succinate dehydrogenase flavoprotein subunit	-
CLC90_RS17550	66297..66653	-	357	WP_126940178.1	hypothetical protein	-
CLC90_RS17555	67407..67607	-	201	WP_126940179.1	hypothetical protein	-
CLC90_RS17560	67650..68156	-	507	WP_126940180.1	antirestriction protein ArdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80843	80843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T111428 WP_038251855.1 NZ_CP032236:c63204-62881 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T111428 NZ_CP032236:c63204-62881 [Yersinia ruckeri]
ATGGAGTATCTAGAATTCATTGAGACCCCCGTATTTAGCAGGCGAAGAGCTGAACTGCTGCCAGATGATGACTTCAGAGC
TTTACAGGAGCATCTTCTTAAAAACCATGATCTTGGCGACACAATCAGTAAAACAGGTGGATGTAAGAAAATCCGCTGGA
GTCGCGAAGGCATGGGGAAGCGGGGGGGAGTAAGAGTTATTTATTACGTCATAACCAAATCGGGACGCCTTTATCTGCTA
CTGGTCTATCCCAAAAACGAAAAAGACGATCTGACTGAAGCCGAAAAAGCTGTCCTGAAATCGATAAGTCAGCAAATCGA
ATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT111428 WP_004720859.1 NZ_CP032236:c62835-62539 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT111428 NZ_CP032236:c62835-62539 [Yersinia ruckeri]
ATGAAAACTGAACTGTTTAATGATCTCGTTGAAAGTATGCAACAGATGGTTGCTATTGAGAAAGGTACGCTCTCTGTTCC
AGCGGAAAACATCCATCGTCATCGACTTCCAGATGTAAAATCAATGCGTGAAGCGAGCGGTCTTAAGCAAAATGAATTTG
CGGATGTAGTGGGGGTTTCTGCTTCTCTGCTCCAGAGCTGGGAACAGCATCGTCGTATTCCATCAGGTTCATCTCTAAAG
TTGTTGATGATGATAGAGCGTGACCCAGCTGTTATAGACGCTTTACGAACGCTTTAG

Structures

Toxin

• AlphaFold DB: A0A380S9H4