Detailed information of TA system TA111422

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 909..1179
• Replicon: plasmid unnamed4
• Accession: NZ_CP032226
• Organism: Klebsiella pneumoniae strain AR_0046

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM425_RS30350
• Protein ID: WP_001312861.1
• Coordinates: 1021..1179 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	909..972 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM425_RS28275	22..741	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	753..977	+	225	NuclAT_0	-	-
-	753..977	+	225	NuclAT_0	-	-
-	753..977	+	225	NuclAT_0	-	-
-	753..977	+	225	NuclAT_0	-	-
-	909..972	-	64	-	-	Antitoxin
AM425_RS30350	1021..1179	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM425_RS28285	1537..1962	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AM425_RS28290	1959..2309	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
AM425_RS28295	2340..3953	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
AM425_RS30355	4038..4242	-	205	Protein_6	pilus protein	-
AM425_RS28315	4634..4930	+	297	WP_001272251.1	hypothetical protein	-
AM425_RS28320	5041..5862	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15	-	1..85410	85410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T111422 WP_001312861.1 NZ_CP032226:1021-1179 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T111422 NZ_CP032226:1021-1179 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT111422 NZ_CP032226:c972-909 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin