Detailed information of TA system TA111378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2331..2961
• Replicon: plasmid unnamed1
• Accession: NZ_CP032206
• Organism: Klebsiella pneumoniae strain AR_0109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3V9PY09
• Locus tag: AM488_RS00015
• Protein ID: WP_001708474.1
• Coordinates: 2626..2961 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM488_RS00010
• Protein ID: WP_042194958.1
• Coordinates: 2331..2621 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM488_RS00005	625..2298	+	1674	WP_064323378.1	hypothetical protein	-
AM488_RS00010	2331..2621	-	291	WP_042194958.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
AM488_RS00015	2626..2961	-	336	WP_001708474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM488_RS00020	3174..3560	-	387	WP_042194962.1	hypothetical protein	-
AM488_RS00025	3610..3936	-	327	WP_064323358.1	hypothetical protein	-
AM488_RS00030	4251..4811	-	561	WP_064323359.1	hypothetical protein	-
AM488_RS00035	4829..5179	-	351	WP_064323360.1	hypothetical protein	-
AM488_RS00040	5176..5445	-	270	WP_102786051.1	hypothetical protein	-
AM488_RS00045	5459..5836	-	378	WP_052957584.1	hypothetical protein	-
AM488_RS00050	5972..7936	-	1965	WP_064323362.1	DUF87 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42202	42202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (20-94)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12615.30 Da
• Isoelectric Point: 5.2407

>T111378 WP_001708474.1 NZ_CP032206:c2961-2626 [Klebsiella pneumoniae]
MEYYEFVETSVFTREMKTLLSDDEYKEFQTFLIENPEAGDLIVGTGGCRKVRWSRQGTGKSSGVRAIYYFYNPAGRLYML
IIYPKSEKDSLTAAEKNQLKAVVAGFKGEEG

Nucleotide

• Length: 336 bp

>T111378 NZ_CP032206:c2961-2626 [Klebsiella pneumoniae]
ATGGAATACTACGAGTTTGTAGAAACCTCTGTATTCACGCGAGAGATGAAAACTCTACTCAGTGATGATGAATACAAAGA
GTTTCAAACGTTCCTCATCGAGAACCCGGAGGCGGGTGATCTGATTGTAGGAACTGGCGGTTGTCGTAAGGTTAGATGGT
CACGGCAGGGAACAGGTAAAAGTTCAGGTGTCAGGGCTATTTACTACTTCTACAACCCGGCAGGCCGCTTATACATGCTT
ATCATCTATCCGAAGAGTGAGAAAGATAGTTTGACGGCAGCAGAGAAAAACCAGCTCAAAGCTGTTGTAGCGGGTTTTAA
AGGTGAAGAGGGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10909.66 Da
• Isoelectric Point: 10.3049

>AT111378 WP_042194958.1 NZ_CP032206:c2621-2331 [Klebsiella pneumoniae]
MRKELFDDLIASAKEAVEIHKGLREPARVTRFERPDVKAIRAKVHMKQDDFASLIGVSSSLVQSWEQGLRTPAGAALKLL
RVIERKPEFVQELREA

Nucleotide

• Length: 291 bp

>AT111378 NZ_CP032206:c2621-2331 [Klebsiella pneumoniae]
ATGCGTAAAGAGCTTTTTGATGATCTGATTGCTTCTGCTAAGGAAGCTGTAGAAATCCATAAGGGTTTACGTGAGCCAGC
GCGGGTTACCCGTTTTGAACGTCCAGATGTAAAAGCTATACGGGCTAAGGTTCATATGAAACAAGATGATTTTGCCAGCC
TGATCGGGGTTAGCTCTTCTCTTGTTCAGTCTTGGGAACAGGGGTTAAGAACTCCGGCAGGTGCAGCGCTAAAGCTGTTG
CGTGTCATCGAGCGTAAACCTGAATTTGTTCAGGAACTACGCGAAGCGTAA

Structures

Toxin

• AlphaFold DB: A0A3V9PY09