Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3960375..3960937 Replicon chromosome
Accession NZ_CP032204
Organism Escherichia coli strain AR_0013

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag AM340_RS20125 Protein ID WP_000605676.1
Coordinates 3960659..3960937 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AM340_RS20120 Protein ID WP_000781370.1
Coordinates 3960375..3960659 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM340_RS20105 3955734..3958781 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
AM340_RS20110 3958794..3959678 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
AM340_RS20115 3959671..3960324 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
AM340_RS20120 3960375..3960659 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
AM340_RS20125 3960659..3960937 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM340_RS20130 3961123..3962133 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
AM340_RS20135 3962267..3963964 - 1698 WP_000433462.1 malate dehydrogenase -
AM340_RS20140 3964121..3964258 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AM340_RS20145 3964360..3964575 - 216 WP_000495767.1 biofilm-dependent modulation protein -
AM340_RS20150 3964920..3965351 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T111365 WP_000605676.1 NZ_CP032204:c3960937-3960659 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T111365 NZ_CP032204:c3960937-3960659 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT111365 WP_000781370.1 NZ_CP032204:c3960659-3960375 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT111365 NZ_CP032204:c3960659-3960375 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCTCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References