Detailed information of TA system TA111346

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128131..128373
• Replicon: plasmid unnamed1
• Accession: NZ_CP032202
• Organism: Escherichia coli strain AR_0086

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM465_RS27360
• Protein ID: WP_001312861.1
• Coordinates: 128215..128373 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128131..128171 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM465_RS27340	123326..125284	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
AM465_RS27345	125339..125773	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AM465_RS27350	125770..126489	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	126501..126687	+	187	NuclAT_0	-	-
-	126501..126687	+	187	NuclAT_0	-	-
-	126501..126687	+	187	NuclAT_0	-	-
-	126501..126687	+	187	NuclAT_0	-	-
AM465_RS27355	126735..128104	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	128131..128171	+	41	NuclAT_1	-	Antitoxin
-	128131..128171	+	41	NuclAT_1	-	Antitoxin
-	128131..128171	+	41	NuclAT_1	-	Antitoxin
-	128131..128171	+	41	NuclAT_1	-	Antitoxin
AM465_RS27360	128215..128373	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM465_RS28165	129064..129270	+	207	WP_000275859.1	hypothetical protein	-
AM465_RS27380	129295..129582	+	288	WP_000107535.1	hypothetical protein	-
AM465_RS27385	129701..130522	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AM465_RS27390	130819..131421	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM465_RS27395	131752..132135	+	384	WP_001151566.1	relaxosome protein TraM	-
AM465_RS27400	132269..132946	+	678	WP_001348626.1	PAS domain-containing protein	-
AM465_RS27405	133034..133261	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..140509	140509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T111346 WP_001312861.1 NZ_CP032202:128215-128373 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T111346 NZ_CP032202:128215-128373 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT111346 NZ_CP032202:128131-128171 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin