Detailed information of TA system TA111340

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18509..18762
• Replicon: plasmid unnamed1
• Accession: NZ_CP032202
• Organism: Escherichia coli strain AR_0086

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: AM465_RS26550
• Protein ID: WP_001336447.1
• Coordinates: 18613..18762 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	18509..18565 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM465_RS26515	14718..15167	+	450	Protein_12	type-F conjugative transfer system pilin acetylase TraX	-
AM465_RS26520	15222..15779	+	558	WP_000139329.1	fertility inhibition protein FinO	-
AM465_RS26525	15931..16134	+	204	WP_001336517.1	hypothetical protein	-
AM465_RS26530	16876..17337	+	462	WP_019842128.1	thermonuclease family protein	-
AM465_RS28140	17440..17824	+	385	Protein_16	hypothetical protein	-
AM465_RS26540	17977..18414	+	438	WP_000872609.1	hypothetical protein	-
-	18509..18565	-	57	NuclAT_2	-	Antitoxin
-	18509..18565	-	57	NuclAT_2	-	Antitoxin
-	18509..18565	-	57	NuclAT_2	-	Antitoxin
-	18509..18565	-	57	NuclAT_2	-	Antitoxin
AM465_RS26550	18613..18762	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM465_RS26555	19047..19304	+	258	WP_000084404.1	replication regulatory protein RepA	-
AM465_RS26565	19540..19614	+	75	WP_001365705.1	RepA leader peptide Tap	-
AM465_RS26570	19607..20464	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
AM465_RS26580	21403..22056	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
AM465_RS28020	22149..22406	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
AM465_RS26585	22339..22740	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..140509	140509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T111340 WP_001336447.1 NZ_CP032202:18613-18762 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T111340 NZ_CP032202:18613-18762 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT111340 NZ_CP032202:c18565-18509 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin