Detailed information of TA system TA111319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 699037..699639
• Replicon: chromosome
• Accession: NZ_CP032201
• Organism: Escherichia coli strain AR_0086

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AM465_RS03705
• Protein ID: WP_000897302.1
• Coordinates: 699328..699639 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM465_RS03700
• Protein ID: WP_000356397.1
• Coordinates: 699037..699327 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM465_RS03660	694344..695273	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
AM465_RS03665	695455..695697	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AM465_RS03670	695987..696835	+	849	WP_001038650.1	hypothetical protein	-
AM465_RS03675	697151..697600	+	450	WP_032140890.1	hypothetical protein	-
AM465_RS03680	697785..698003	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AM465_RS03690	698400..698678	-	279	WP_001296612.1	hypothetical protein	-
AM465_RS03700	699037..699327	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM465_RS03705	699328..699639	-	312	WP_000897302.1	hypothetical protein	Toxin
AM465_RS03710	699868..700776	+	909	WP_001385591.1	alpha/beta hydrolase	-
AM465_RS03715	700840..701781	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM465_RS03720	701826..702263	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM465_RS03725	702260..703132	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AM465_RS03730	703126..703725	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T111319 WP_000897302.1 NZ_CP032201:c699639-699328 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T111319 NZ_CP032201:c699639-699328 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT111319 WP_000356397.1 NZ_CP032201:c699327-699037 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT111319 NZ_CP032201:c699327-699037 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416