Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2231650..2232296 Replicon chromosome
Accession NZ_CP032200
Organism Klebsiella pneumoniae strain AR_0097

Toxin (Protein)


Gene name higB Uniprot ID A0A483JFR4
Locus tag AM476_RS13340 Protein ID WP_004188313.1
Coordinates 2231650..2231997 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A483GM64
Locus tag AM476_RS13345 Protein ID WP_004188315.1
Coordinates 2231997..2232296 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM476_RS13330 2227576..2229009 + 1434 WP_002920564.1 glycogen synthase GlgA -
AM476_RS13335 2229027..2231474 + 2448 WP_002920561.1 glycogen phosphorylase -
AM476_RS13340 2231650..2231997 + 348 WP_004188313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM476_RS13345 2231997..2232296 + 300 WP_004188315.1 XRE family transcriptional regulator Antitoxin
AM476_RS13350 2232359..2233867 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
AM476_RS13355 2234072..2234401 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
AM476_RS13360 2234452..2235282 + 831 WP_004188317.1 rhomboid family intramembrane serine protease GlpG -
AM476_RS13365 2235332..2236090 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-78)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13548.57 Da        Isoelectric Point: 6.2327

>T111303 WP_004188313.1 NZ_CP032200:2231650-2231997 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T111303 NZ_CP032200:2231650-2231997 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10873.64 Da        Isoelectric Point: 10.1464

>AT111303 WP_004188315.1 NZ_CP032200:2231997-2232296 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAAWG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT111303 NZ_CP032200:2231997-2232296 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483JFR4

Antitoxin

Source ID Structure
AlphaFold DB A0A483GM64

References