Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 433136..433769 Replicon chromosome
Accession NZ_CP032200
Organism Klebsiella pneumoniae strain AR_0097

Toxin (Protein)


Gene name higB Uniprot ID A0A9J6S5A1
Locus tag AM476_RS04530 Protein ID WP_004893639.1
Coordinates 433452..433769 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AM476_RS04525 Protein ID WP_104467732.1
Coordinates 433136..433459 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM476_RS04495 429380..429628 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AM476_RS04500 429646..429987 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AM476_RS04505 430018..431133 - 1116 WP_074180844.1 MBL fold metallo-hydrolase -
AM476_RS04510 431313..431894 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
AM476_RS04515 431894..432262 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AM476_RS04520 432382..433035 + 654 Protein_390 oxygen-insensitive NAD(P)H nitroreductase -
AM476_RS04525 433136..433459 - 324 WP_104467732.1 helix-turn-helix transcriptional regulator Antitoxin
AM476_RS04530 433452..433769 - 318 WP_004893639.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM476_RS04535 433954..434997 - 1044 WP_023279204.1 DUF2157 domain-containing protein -
AM476_RS04545 435667..436533 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
AM476_RS04550 436642..438069 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12100.35 Da        Isoelectric Point: 11.2767

>T111295 WP_004893639.1 NZ_CP032200:c433769-433452 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T111295 NZ_CP032200:c433769-433452 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11912.62 Da        Isoelectric Point: 10.0437

>AT111295 WP_104467732.1 NZ_CP032200:c433459-433136 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVYFTNALGGYSLTGAN

Download         Length: 324 bp

>AT111295 NZ_CP032200:c433459-433136 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTACTTTACTAACGCGCTCGGTGGCTATTCACTTACCGGCGCAAA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References