Detailed information of TA system TA111284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4415505..4416145
• Replicon: chromosome
• Accession: NZ_CP032185
• Organism: Klebsiella pneumoniae strain AR_0075

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: AM454_RS22430
• Protein ID: WP_016529833.1
• Coordinates: 4415798..4416145 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM454_RS22425
• Protein ID: WP_040181955.1
• Coordinates: 4415505..4415798 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM454_RS22405	4411704..4412462	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
AM454_RS22410	4412512..4413342	-	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
AM454_RS22415	4413393..4413722	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AM454_RS22420	4413927..4415435	+	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
AM454_RS22425	4415505..4415798	-	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
AM454_RS22430	4415798..4416145	-	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM454_RS22435	4416321..4418768	-	2448	WP_002920561.1	glycogen phosphorylase	-
AM454_RS22440	4418786..4420219	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T111284 WP_016529833.1 NZ_CP032185:c4416145-4415798 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T111284 NZ_CP032185:c4416145-4415798 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT111284 WP_040181955.1 NZ_CP032185:c4415798-4415505 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT111284 NZ_CP032185:c4415798-4415505 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Toxin

• AlphaFold DB: W9BBY1