Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3278907..3279504 Replicon chromosome
Accession NZ_CP032178
Organism Klebsiella pneumoniae strain AR_0135

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AM382_RS17175 Protein ID WP_004142563.1
Coordinates 3278907..3279224 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AM382_RS17180 Protein ID WP_004142561.1
Coordinates 3279217..3279504 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM382_RS17155 3274610..3276037 - 1428 WP_004176980.1 MFS transporter -
AM382_RS17160 3276146..3277012 + 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
AM382_RS17170 3277679..3278722 + 1044 WP_032419264.1 DUF2157 domain-containing protein -
AM382_RS17175 3278907..3279224 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM382_RS17180 3279217..3279504 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AM382_RS17185 3279769..3280422 - 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
AM382_RS17190 3280542..3280910 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AM382_RS17195 3280910..3281491 - 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
AM382_RS17200 3281670..3282785 + 1116 WP_162900879.1 MBL fold metallo-hydrolase -
AM382_RS17205 3282816..3283157 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AM382_RS17210 3283175..3283423 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T111260 WP_004142563.1 NZ_CP032178:3278907-3279224 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T111260 NZ_CP032178:3278907-3279224 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT111260 WP_004142561.1 NZ_CP032178:3279217-3279504 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT111260 NZ_CP032178:3279217-3279504 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References