Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1698536..1699182 Replicon chromosome
Accession NZ_CP032175
Organism Klebsiella pneumoniae strain AR_0160

Toxin (Protein)


Gene name higB Uniprot ID A0A486RBU5
Locus tag AM406_RS09785 Protein ID WP_032410616.1
Coordinates 1698835..1699182 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag AM406_RS09780 Protein ID WP_002920557.1
Coordinates 1698536..1698835 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM406_RS09760 1694742..1695500 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
AM406_RS09765 1695550..1696380 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
AM406_RS09770 1696431..1696760 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
AM406_RS09775 1696965..1698473 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
AM406_RS09780 1698536..1698835 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
AM406_RS09785 1698835..1699182 - 348 WP_032410616.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM406_RS09790 1699358..1701805 - 2448 WP_002920561.1 glycogen phosphorylase -
AM406_RS09795 1701823..1703256 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.55 Da        Isoelectric Point: 5.6802

>T111244 WP_032410616.1 NZ_CP032175:c1699182-1698835 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T111244 NZ_CP032175:c1699182-1698835 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT111244 WP_002920557.1 NZ_CP032175:c1698835-1698536 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT111244 NZ_CP032175:c1698835-1698536 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RBU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References