Detailed information of TA system TA111199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 85573..86300
• Replicon: plasmid unnamed1
• Accession: NZ_CP032164
• Organism: Klebsiella pneumoniae strain XJ-K1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: D3H40_RS27750
• Protein ID: WP_011251285.1
• Coordinates: 85573..85884 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3H40_RS27755
• Protein ID: WP_011251286.1
• Coordinates: 85881..86300 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3H40_RS27705	80584..81094	+	511	Protein_90	hypothetical protein	-
D3H40_RS27710	81084..81524	+	441	WP_011251275.1	hypothetical protein	-
D3H40_RS27720	81949..82905	-	957	WP_011251280.1	DsbA family protein	-
D3H40_RS27725	82965..83306	-	342	WP_011251281.1	hypothetical protein	-
D3H40_RS27730	83320..83631	-	312	WP_011251282.1	hypothetical protein	-
D3H40_RS27735	83648..84082	-	435	Protein_95	thioredoxin fold domain-containing protein	-
D3H40_RS27740	84099..84796	-	698	Protein_96	IS1 family transposase	-
D3H40_RS27745	84910..85368	+	459	Protein_97	DDE-type integrase/transposase/recombinase	-
D3H40_RS27750	85573..85884	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
D3H40_RS27755	85881..86300	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
D3H40_RS27765	86447..87415	+	969	WP_074428168.1	IS5 family transposase	-
D3H40_RS27770	87517..87852	-	336	Protein_101	conjugal transfer protein TraI	-
D3H40_RS27775	87866..88654	-	789	WP_040217257.1	hypothetical protein	-
D3H40_RS27780	88675..89118	-	444	WP_048333569.1	hypothetical protein	-
D3H40_RS30695	89714..90469	+	756	Protein_104	hypothetical protein	-
D3H40_RS27790	90662..91132	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..207409	207409
	-	inside	IScluster/Tn	-	-	78664..102971	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T111199 WP_011251285.1 NZ_CP032164:85573-85884 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T111199 NZ_CP032164:85573-85884 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT111199 WP_011251286.1 NZ_CP032164:85881-86300 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT111199 NZ_CP032164:85881-86300 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1