Detailed information of TA system TA111158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4499318..4499920
• Replicon: chromosome
• Accession: NZ_CP032145
• Organism: Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: D3F09_RS21980
• Protein ID: WP_000897305.1
• Coordinates: 4499318..4499629 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3F09_RS21985
• Protein ID: WP_000356395.1
• Coordinates: 4499630..4499920 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3F09_RS21960	4494360..4494797	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D3F09_RS21965	4494842..4495783	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D3F09_RS21970	4496199..4498013	+	1815	WP_001550352.1	hypothetical protein	-
D3F09_RS21975	4498181..4499089	-	909	WP_001550353.1	alpha/beta hydrolase	-
D3F09_RS21980	4499318..4499629	+	312	WP_000897305.1	hypothetical protein	Toxin
D3F09_RS21985	4499630..4499920	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
D3F09_RS21990	4500005..4500226	+	222	WP_001550354.1	hypothetical protein	-
D3F09_RS21995	4500278..4500556	+	279	WP_001315112.1	hypothetical protein	-
D3F09_RS22005	4500975..4501193	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
D3F09_RS22010	4501412..4501654	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
D3F09_RS22015	4501836..4502765	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
D3F09_RS22020	4502762..4503397	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D3F09_RS22025	4503394..4504296	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T111158 WP_000897305.1 NZ_CP032145:4499318-4499629 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T111158 NZ_CP032145:4499318-4499629 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT111158 WP_000356395.1 NZ_CP032145:4499630-4499920 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT111158 NZ_CP032145:4499630-4499920 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7