Detailed information of TA system TA111151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3078943..3079670
• Replicon: chromosome
• Accession: NZ_CP032145
• Organism: Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: D3F09_RS14985
• Protein ID: WP_000547555.1
• Coordinates: 3079359..3079670 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3F09_RS14980
• Protein ID: WP_000126294.1
• Coordinates: 3078943..3079362 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3F09_RS14960	3074028..3074555	-	528	WP_001078777.1	electron transport protein HydN	-
D3F09_RS14965	3074704..3075714	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
D3F09_RS14970	3075974..3077431	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
D3F09_RS14975	3077440..3078864	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
D3F09_RS14980	3078943..3079362	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
D3F09_RS14985	3079359..3079670	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
D3F09_RS14990	3079837..3080307	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
D3F09_RS14995	3080300..3080710	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
D3F09_RS15000	3080707..3081474	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
D3F09_RS15005	3081474..3082016	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
D3F09_RS15010	3082026..3083735	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T111151 WP_000547555.1 NZ_CP032145:c3079670-3079359 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T111151 NZ_CP032145:c3079670-3079359 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT111151 WP_000126294.1 NZ_CP032145:c3079362-3078943 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT111151 NZ_CP032145:c3079362-3078943 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2