Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2276985..2277487 Replicon chromosome
Accession NZ_CP032145
Organism Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D3F09_RS11135 Protein ID WP_000767829.1
Coordinates 2276985..2277239 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D3F09_RS11140 Protein ID WP_001259255.1
Coordinates 2277236..2277487 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3F09_RS11105 2272000..2272227 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
D3F09_RS11110 2272241..2273299 - 1059 WP_023568741.1 transport protein YeeE -
D3F09_RS11115 2273478..2274836 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D3F09_RS11120 2274826..2274888 - 63 WP_010723108.1 membrane protein YoeI -
D3F09_RS11125 2275103..2276032 - 930 WP_000803351.1 LysR family transcriptional regulator -
D3F09_RS11130 2276078..2276902 - 825 WP_000754737.1 SDR family oxidoreductase -
D3F09_RS11135 2276985..2277239 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D3F09_RS11140 2277236..2277487 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D3F09_RS11145 2277770..2277820 + 51 WP_071593552.1 his operon leader peptide -
D3F09_RS11150 2277966..2278865 + 900 WP_000131789.1 ATP phosphoribosyltransferase -
D3F09_RS11155 2278871..2280175 + 1305 WP_039267630.1 histidinol dehydrogenase -
D3F09_RS11160 2280172..2281242 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
D3F09_RS11165 2281242..2282309 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T111150 WP_000767829.1 NZ_CP032145:c2277239-2276985 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111150 NZ_CP032145:c2277239-2276985 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111150 WP_001259255.1 NZ_CP032145:c2277487-2277236 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111150 NZ_CP032145:c2277487-2277236 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References