Detailed information of TA system TA111135

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1486819..1487190
• Replicon: chromosome
• Accession: NZ_CP032145
• Organism: Escherichia coli strain 15.TR.026_OXA

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: D3F09_RS07145
• Protein ID: WP_001317028.1
• Coordinates: 1486996..1487190 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1486819..1486997 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3F09_RS07120	1482774..1484147	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
D3F09_RS07125	1484276..1485211	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
D3F09_RS07130	1485263..1486498	-	1236	WP_001551035.1	site-specific integrase	-
D3F09_RS07135	1486500..1486715	-	216	WP_000079604.1	excisionase XisR	-
D3F09_RS07140	1486815..1487003	-	189	WP_001551036.1	DUF1187 family protein	-
-	1486819..1486997	+	179	NuclAT_0	-	Antitoxin
-	1486819..1486997	+	179	NuclAT_0	-	Antitoxin
-	1486819..1486997	+	179	NuclAT_0	-	Antitoxin
-	1486819..1486997	+	179	NuclAT_0	-	Antitoxin
D3F09_RS07145	1486996..1487190	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
D3F09_RS07150	1487247..1488056	-	810	WP_001551037.1	recombination protein RecT	-
D3F09_RS07155	1488049..1490700	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
D3F09_RS07160	1490802..1491077	-	276	WP_001314664.1	hypothetical protein	-
D3F09_RS07165	1491152..1491322	-	171	WP_001551041.1	phage protein	-
D3F09_RS07170	1491322..1491543	-	222	WP_000560221.1	killing protein KilR	-
D3F09_RS07175	1491964..1492116	-	153	WP_001551042.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1485263..1532553	47290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T111135 WP_001317028.1 NZ_CP032145:c1487190-1486996 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T111135 NZ_CP032145:c1487190-1486996 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT111135 NZ_CP032145:1486819-1486997 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin