Detailed information of TA system TA111124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1276..1927
• Replicon: plasmid p1_010052
• Accession: NZ_CP032138
• Organism: Acinetobacter sp. WCHAc010052

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BC17
• Locus tag: CDG61_RS00020
• Protein ID: WP_004967307.1
• Coordinates: 1571..1927 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG61_RS00015
• Protein ID: WP_001140620.1
• Coordinates: 1276..1578 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG61_RS00005	107..718	-	612	WP_015060246.1	recombinase family protein	-
CDG61_RS00010	919..1194	+	276	WP_004967304.1	hypothetical protein	-
CDG61_RS00015	1276..1578	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
CDG61_RS00020	1571..1927	-	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG61_RS00030	2148..2984	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
CDG61_RS00035	2984..3787	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
CDG61_RS00040	3967..4782	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
CDG61_RS00045	4917..4988	-	72	Protein_7	type II toxin-antitoxin system HipA family toxin	-
CDG61_RS00050	5111..6220	-	1110	WP_004968110.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / tet(39) / msr(E) / mph(E)	-	1..16399	16399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13601.62 Da
• Isoelectric Point: 6.4648

>T111124 WP_004967307.1 NZ_CP032138:c1927-1571 [Acinetobacter sp. WCHAc010052]
MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGSKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T111124 NZ_CP032138:c1927-1571 [Acinetobacter sp. WCHAc010052]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT111124 WP_001140620.1 NZ_CP032138:c1578-1276 [Acinetobacter sp. WCHAc010052]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT111124 NZ_CP032138:c1578-1276 [Acinetobacter sp. WCHAc010052]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7T4TW67