Detailed information of TA system TA111120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5361664..5362259
• Replicon: chromosome
• Accession: NZ_CP032126
• Organism: Pseudomonas aeruginosa strain PAO1161

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: D3C65_RS25500
• Protein ID: WP_003113526.1
• Coordinates: 5361981..5362259 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3C65_RS25495
• Protein ID: WP_003113527.1
• Coordinates: 5361664..5361969 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3C65_RS25460	5356777..5357067	-	291	WP_033992920.1	DUF5447 family protein	-
D3C65_RS25470	5357279..5357551	-	273	WP_003115921.1	hypothetical protein	-
D3C65_RS25480	5358081..5359178	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
D3C65_RS25485	5359181..5360230	+	1050	WP_003113529.1	serine/threonine protein kinase	-
D3C65_RS25490	5360227..5361300	+	1074	WP_003113528.1	serine/threonine protein kinase	-
D3C65_RS25495	5361664..5361969	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
D3C65_RS25500	5361981..5362259	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3C65_RS25510	5362588..5364816	+	2229	WP_003113525.1	TonB-dependent receptor	-
D3C65_RS25515	5364886..5365533	-	648	WP_003095021.1	carbonate dehydratase	-
D3C65_RS25520	5365595..5366833	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T111120 WP_003113526.1 NZ_CP032126:c5362259-5361981 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T111120 NZ_CP032126:c5362259-5361981 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT111120 WP_003113527.1 NZ_CP032126:c5361969-5361664 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT111120 NZ_CP032126:c5361969-5361664 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3