Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 68953..69599 Replicon plasmid pL25922_3
Accession NZ_CP032086
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID A0A7R6ZH56
Locus tag D1792_RS25105 Protein ID WP_000269913.1
Coordinates 69252..69599 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag D1792_RS25100 Protein ID WP_001259436.1
Coordinates 68953..69252 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS25070 64787..65359 - 573 WP_000137931.1 helix-turn-helix transcriptional regulator -
D1792_RS25075 65933..66124 + 192 WP_000183352.1 hypothetical protein -
D1792_RS25080 66121..67263 + 1143 WP_000854799.1 ORF6N domain-containing protein -
D1792_RS25085 67274..67462 - 189 WP_000986262.1 hypothetical protein -
D1792_RS25090 68073..68345 - 273 WP_000148349.1 helix-turn-helix domain-containing protein -
D1792_RS25095 68409..68789 - 381 WP_000061764.1 hypothetical protein -
D1792_RS25100 68953..69252 - 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
D1792_RS25105 69252..69599 - 348 WP_000269913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D1792_RS25110 69844..70107 - 264 WP_000424604.1 hypothetical protein -
D1792_RS25115 70131..70418 - 288 WP_000356589.1 hypothetical protein -
D1792_RS25120 71062..71898 - 837 WP_001139598.1 hypothetical protein -
D1792_RS25125 72224..72778 - 555 WP_001199265.1 recombinase family protein -
D1792_RS25130 72842..73807 + 966 WP_136858490.1 phage tail protein -
D1792_RS25135 73810..74343 + 534 WP_000972173.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94204 94204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13486.53 Da        Isoelectric Point: 9.8723

>T111091 WP_000269913.1 NZ_CP032086:c69599-69252 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

>T111091 NZ_CP032086:c69599-69252 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT111091 WP_001259436.1 NZ_CP032086:c69252-68953 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp

>AT111091 NZ_CP032086:c69252-68953 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7R6ZH56

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References