Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 20465..21111 Replicon plasmid pL25922_3
Accession NZ_CP032086
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID A0A7R6ZH56
Locus tag D1792_RS24770 Protein ID WP_000269913.1
Coordinates 20764..21111 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag D1792_RS24765 Protein ID WP_001259436.1
Coordinates 20465..20764 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS24735 16300..16872 - 573 WP_000137931.1 helix-turn-helix transcriptional regulator -
D1792_RS24740 17446..17637 + 192 WP_000183352.1 hypothetical protein -
D1792_RS24745 17634..18776 + 1143 WP_000854799.1 ORF6N domain-containing protein -
D1792_RS24750 18787..18975 - 189 WP_000986262.1 hypothetical protein -
D1792_RS24755 19585..19857 - 273 WP_000148349.1 helix-turn-helix domain-containing protein -
D1792_RS24760 19921..20301 - 381 WP_000061764.1 hypothetical protein -
D1792_RS24765 20465..20764 - 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
D1792_RS24770 20764..21111 - 348 WP_000269913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D1792_RS24775 21356..21619 - 264 WP_000424604.1 hypothetical protein -
D1792_RS24780 21643..21930 - 288 WP_000356589.1 hypothetical protein -
D1792_RS24785 22574..23410 - 837 WP_001139598.1 hypothetical protein -
D1792_RS24790 23736..24290 - 555 WP_001199265.1 recombinase family protein -
D1792_RS24795 24456..25382 + 927 Protein_37 phage tail protein -
D1792_RS24800 25384..25911 + 528 WP_000972103.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94204 94204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13486.53 Da        Isoelectric Point: 9.8723

>T111090 WP_000269913.1 NZ_CP032086:c21111-20764 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

>T111090 NZ_CP032086:c21111-20764 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT111090 WP_001259436.1 NZ_CP032086:c20764-20465 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp

>AT111090 NZ_CP032086:c20764-20465 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7R6ZH56

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References