Detailed information of TA system TA111080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3377542..3378144
• Replicon: chromosome
• Accession: NZ_CP032085
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: D1792_RS16290
• Protein ID: WP_000897302.1
• Coordinates: 3377542..3377853 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D1792_RS16295
• Protein ID: WP_000356397.1
• Coordinates: 3377854..3378144 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1792_RS16265	3373455..3374054	+	600	WP_001443191.1	glucose-1-phosphatase	-
D1792_RS16270	3374048..3374920	+	873	WP_000920762.1	virulence factor BrkB family protein	-
D1792_RS16275	3374917..3375354	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D1792_RS16280	3375399..3376340	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D1792_RS16285	3376404..3377312	-	909	WP_001305059.1	alpha/beta hydrolase	-
D1792_RS16290	3377542..3377853	+	312	WP_000897302.1	hypothetical protein	Toxin
D1792_RS16295	3377854..3378144	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
D1792_RS16300	3378503..3378781	+	279	WP_001296612.1	hypothetical protein	-
D1792_RS16305	3379177..3379395	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
D1792_RS16310	3379611..3380540	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
D1792_RS16315	3380537..3381172	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D1792_RS16320	3381169..3382071	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T111080 WP_000897302.1 NZ_CP032085:3377542-3377853 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T111080 NZ_CP032085:3377542-3377853 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT111080 WP_000356397.1 NZ_CP032085:3377854-3378144 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT111080 NZ_CP032085:3377854-3378144 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416