Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1947519..1948246 Replicon chromosome
Accession NZ_CP032085
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag D1792_RS09475 Protein ID WP_000547555.1
Coordinates 1947935..1948246 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag D1792_RS09470 Protein ID WP_000126293.1
Coordinates 1947519..1947938 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1792_RS09455 1943682..1945934 - 2253 WP_001107659.1 carbamoyltransferase HypF -
D1792_RS09460 1946087..1946614 - 528 WP_001078777.1 electron transport protein HydN -
D1792_RS09465 1946999..1947427 + 429 WP_000536064.1 hypothetical protein -
D1792_RS09470 1947519..1947938 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
D1792_RS09475 1947935..1948246 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
D1792_RS09480 1948409..1949164 - 756 WP_001305268.1 hypothetical protein -
D1792_RS09485 1949207..1949677 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
D1792_RS09490 1949670..1950080 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
D1792_RS09495 1950077..1950844 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
D1792_RS09500 1950844..1951386 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
D1792_RS09505 1951396..1953105 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T111075 WP_000547555.1 NZ_CP032085:c1948246-1947935 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T111075 NZ_CP032085:c1948246-1947935 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT111075 WP_000126293.1 NZ_CP032085:c1947938-1947519 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT111075 NZ_CP032085:c1947938-1947519 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References