Detailed information of TA system TA111044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4739385..4739987
• Replicon: chromosome
• Accession: NZ_CP032078
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: D2195_RS22930
• Protein ID: WP_000897305.1
• Coordinates: 4739676..4739987 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D2195_RS22925
• Protein ID: WP_000356397.1
• Coordinates: 4739385..4739675 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS22905 (4735887)	4735887..4736789	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
D2195_RS22910 (4736786)	4736786..4737421	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D2195_RS22915 (4737418)	4737418..4738347	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
D2195_RS22920 (4738562)	4738562..4738780	-	219	WP_001295676.1	CopG family transcriptional regulator	-
D2195_RS22925 (4739385)	4739385..4739675	-	291	WP_000356397.1	NadS family protein	Antitoxin
D2195_RS22930 (4739676)	4739676..4739987	-	312	WP_000897305.1	hypothetical protein	Toxin
D2195_RS22935 (4740216)	4740216..4741124	+	909	WP_001318161.1	alpha/beta hydrolase	-
D2195_RS22940 (4741188)	4741188..4742129	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D2195_RS22945 (4742174)	4742174..4742611	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
D2195_RS22950 (4742608)	4742608..4743480	-	873	WP_000920762.1	virulence factor BrkB family protein	-
D2195_RS22955 (4743474)	4743474..4744073	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T111044 WP_000897305.1 NZ_CP032078:c4739987-4739676 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T111044 NZ_CP032078:c4739987-4739676 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT111044 WP_000356397.1 NZ_CP032078:c4739675-4739385 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT111044 NZ_CP032078:c4739675-4739385 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7