111010

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4126557..4127054 Replicon chromosome
Accession NZ_CP032073
Organism Escherichia coli strain CT29

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D2194_RS19930 Protein ID WP_001173343.1
Coordinates 4126557..4126730 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag D2194_RS19935 Protein ID WP_000398619.1
Coordinates 4126758..4127054 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2194_RS19920 (4123227) 4123227..4124684 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
D2194_RS19925 (4124921) 4124921..4126438 + 1518 WP_001295074.1 lysine--tRNA ligase -
D2194_RS19930 (4126557) 4126557..4126730 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D2194_RS19935 (4126758) 4126758..4127054 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D2194_RS19940 (4127281) 4127281..4127553 - 273 WP_096991230.1 GNAT family N-acetyltransferase -
D2194_RS19945 (4127565) 4127565..4127795 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
D2194_RS19950 (4127976) 4127976..4129607 + 1632 WP_001216477.1 sensor histidine kinase -
D2194_RS19955 (4129604) 4129604..4130323 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4118414..4127054 8640


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T111010 WP_001173343.1 NZ_CP032073:c4126730-4126557 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T111010 NZ_CP032073:c4126730-4126557 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT111010 WP_000398619.1 NZ_CP032073:c4127054-4126758 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT111010 NZ_CP032073:c4127054-4126758 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References