Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3605720..3606414 Replicon chromosome
Accession NZ_CP032073
Organism Escherichia coli strain CT29

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag D2194_RS17415 Protein ID WP_001263493.1
Coordinates 3605720..3606118 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag D2194_RS17420 Protein ID WP_000554757.1
Coordinates 3606121..3606414 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3601380) 3601380..3601460 - 81 NuclAT_9 - -
- (3601380) 3601380..3601460 - 81 NuclAT_9 - -
- (3601380) 3601380..3601460 - 81 NuclAT_9 - -
- (3601380) 3601380..3601460 - 81 NuclAT_9 - -
D2194_RS17385 (3600720) 3600720..3601964 - 1245 WP_000189541.1 esterase FrsA -
D2194_RS17390 (3602056) 3602056..3602514 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
D2194_RS17395 (3602775) 3602775..3604232 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
D2194_RS17400 (3604289) 3604289..3604810 - 522 Protein_3443 peptide chain release factor H -
D2194_RS17405 (3604809) 3604809..3605012 - 204 Protein_3444 RtcB family protein -
D2194_RS17410 (3605258) 3605258..3605710 - 453 WP_180759961.1 GNAT family N-acetyltransferase -
D2194_RS17415 (3605720) 3605720..3606118 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
D2194_RS17420 (3606121) 3606121..3606414 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
D2194_RS17425 (3606466) 3606466..3607521 - 1056 WP_001226164.1 DNA polymerase IV -
D2194_RS17430 (3607592) 3607592..3608377 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
D2194_RS17435 (3608349) 3608349..3610061 + 1713 Protein_3450 flagellar biosynthesis protein FlhA -
D2194_RS17440 (3610285) 3610285..3610782 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3604758..3638079 33321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T111009 WP_001263493.1 NZ_CP032073:c3606118-3605720 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T111009 NZ_CP032073:c3606118-3605720 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT111009 WP_000554757.1 NZ_CP032073:c3606414-3606121 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT111009 NZ_CP032073:c3606414-3606121 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References