Detailed information of TA system TA110992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 621295..622094
• Replicon: chromosome
• Accession: NZ_CP032073
• Organism: Escherichia coli strain CT29

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D2194_RS03035
• Protein ID: WP_180469665.1
• Coordinates: 621295..621759 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: D2194_RS03040
• Protein ID: WP_212403239.1
• Coordinates: 621759..622094 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2194_RS03005 (616296)	616296..616730	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
D2194_RS03010 (616748)	616748..617626	-	879	WP_180469662.1	PTS N-acetylgalactosamine transporter subunit IID	-
D2194_RS03015 (617616)	617616..618395	-	780	WP_212403236.1	PTS N-acetylgalactosamine transporter subunit IIC	-
D2194_RS03020 (618406)	618406..618879	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D2194_RS03025 (618902)	618902..620182	-	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D2194_RS03030 (620431)	620431..621240	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D2194_RS03035 (621295)	621295..621759	-	465	WP_180469665.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D2194_RS03040 (621759)	621759..622094	-	336	WP_212403239.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
D2194_RS03045 (622243)	622243..623814	-	1572	WP_032181387.1	galactarate dehydratase	-
D2194_RS03050 (624189)	624189..625523	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
D2194_RS03055 (625539)	625539..626309	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	611894..632864	20970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17793.18 Da
• Isoelectric Point: 9.4947

>T110992 WP_180469665.1 NZ_CP032073:c621759-621295 [Escherichia coli]
MDFLQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T110992 NZ_CP032073:c621759-621295 [Escherichia coli]
ATGGATTTTCTACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12443.11 Da
• Isoelectric Point: 5.1498

>AT110992 WP_212403239.1 NZ_CP032073:c622094-621759 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGRQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT110992 NZ_CP032073:c622094-621759 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTAGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA