Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | I | Classification (family/domain) | hok-sok/- |
Location | 105594..105848 | Replicon | plasmid p.E166-P2 |
Accession | NZ_CP032068 | ||
Organism | Escherichia coli strain E166 |
Toxin (Protein)
Gene name | srnB | Uniprot ID | G9G1E3 |
Locus tag | D2J89_RS24805 | Protein ID | WP_001312851.1 |
Coordinates | 105699..105848 (+) | Length | 50 a.a. |
Antitoxin (RNA)
Gene name | sok | ||
Locus tag | - | ||
Coordinates | 105594..105655 (-) |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
D2J89_RS24780 (102342) | 102342..103088 | + | 747 | WP_000205749.1 | conjugal transfer pilus acetylase TraX | - |
D2J89_RS24785 (103147) | 103147..104007 | + | 861 | WP_000704513.1 | alpha/beta hydrolase | - |
D2J89_RS24790 (104110) | 104110..104670 | + | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
D2J89_RS24795 (104806) | 104806..105018 | + | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
D2J89_RS24800 (105264) | 105264..105338 | + | 75 | Protein_123 | endonuclease | - |
- (105594) | 105594..105655 | - | 62 | NuclAT_1 | - | Antitoxin |
- (105594) | 105594..105655 | - | 62 | NuclAT_1 | - | Antitoxin |
- (105594) | 105594..105655 | - | 62 | NuclAT_1 | - | Antitoxin |
- (105594) | 105594..105655 | - | 62 | NuclAT_1 | - | Antitoxin |
D2J89_RS24805 (105699) | 105699..105848 | + | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin |
D2J89_RS24810 (106132) | 106132..106389 | + | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
D2J89_RS25980 (106406) | 106406..106657 | - | 252 | WP_223195197.1 | replication protein RepA | - |
D2J89_RS25985 (106648) | 106648..106695 | + | 48 | WP_229471593.1 | hypothetical protein | - |
D2J89_RS24820 (106688) | 106688..107170 | + | 483 | WP_001273588.1 | hypothetical protein | - |
D2J89_RS24825 (107163) | 107163..108020 | + | 858 | WP_000774297.1 | incFII family plasmid replication initiator RepA | - |
D2J89_RS24830 (108959) | 108959..109612 | + | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
D2J89_RS24835 (109705) | 109705..109962 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
D2J89_RS24840 (109895) | 109895..110296 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | sitABCD / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 | iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB | 1..163224 | 163224 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5542.67 Da Isoelectric Point: 8.7678
>T110987 WP_001312851.1 NZ_CP032068:105699-105848 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
Download Length: 150 bp
>T110987 NZ_CP032068:105699-105848 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
Antitoxin
Download Length: 62 bp
>AT110987 NZ_CP032068:c105655-105594 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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