Detailed information of TA system TA110956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1488552..1489154
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: D2J89_RS07145
• Protein ID: WP_000897305.1
• Coordinates: 1488843..1489154 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D2J89_RS07140
• Protein ID: WP_000356397.1
• Coordinates: 1488552..1488842 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS07120 (1485054)	1485054..1485956	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
D2J89_RS07125 (1485953)	1485953..1486588	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D2J89_RS07130 (1486585)	1486585..1487514	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
D2J89_RS07135 (1487729)	1487729..1487947	-	219	WP_001295676.1	CopG family transcriptional regulator	-
D2J89_RS07140 (1488552)	1488552..1488842	-	291	WP_000356397.1	NadS family protein	Antitoxin
D2J89_RS07145 (1488843)	1488843..1489154	-	312	WP_000897305.1	hypothetical protein	Toxin
D2J89_RS07150 (1489383)	1489383..1490291	+	909	WP_001318161.1	alpha/beta hydrolase	-
D2J89_RS07155 (1490355)	1490355..1491296	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D2J89_RS07160 (1491341)	1491341..1491778	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
D2J89_RS07165 (1491775)	1491775..1492647	-	873	WP_000920762.1	virulence factor BrkB family protein	-
D2J89_RS07170 (1492641)	1492641..1493240	-	600	WP_212407094.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110956 WP_000897305.1 NZ_CP032066:c1489154-1488843 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110956 NZ_CP032066:c1489154-1488843 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110956 WP_000356397.1 NZ_CP032066:c1488842-1488552 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110956 NZ_CP032066:c1488842-1488552 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7