Detailed information of TA system TA110904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 57310..57961
• Replicon: plasmid pAhaem2126chf
• Accession: NZ_CP031992
• Organism: Acinetobacter haemolyticus strain 2126ch

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2L0I0R9
• Locus tag: Ahae2126ch_RS17365
• Protein ID: WP_019383563.1
• Coordinates: 57310..57666 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Ahae2126ch_RS17370
• Protein ID: WP_001140620.1
• Coordinates: 57659..57961 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ahae2126ch_RS17330	52742..53281	-	540	WP_112987680.1	IS630 family transposase	-
Ahae2126ch_RS17335	53277..53753	-	477	WP_004677047.1	helix-turn-helix domain-containing protein	-
Ahae2126ch_RS17340	54047..54181	+	135	Protein_45	IS481 family transposase	-
Ahae2126ch_RS17345	54253..54633	-	381	Protein_46	hypothetical protein	-
Ahae2126ch_RS17350	54689..55621	-	933	WP_020846310.1	IS5 family transposase	-
Ahae2126ch_RS17355	55676..56134	-	459	Protein_48	hypothetical protein	-
Ahae2126ch_RS17360	56134..56592	-	459	WP_019383562.1	TIR domain-containing protein	-
Ahae2126ch_RS17365	57310..57666	+	357	WP_019383563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Ahae2126ch_RS17370	57659..57961	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
Ahae2126ch_RS17375	58003..58164	-	162	Protein_52	IS6 family transposase	-
Ahae2126ch_RS17380	58258..58650	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
Ahae2126ch_RS17385	58740..60020	+	1281	WP_005093338.1	cation transporter	-
Ahae2126ch_RS17390	60084..60788	+	705	WP_019383564.1	IS6-like element IS1008 family transposase	-
Ahae2126ch_RS17395	60816..61013	-	198	WP_019383565.1	hypothetical protein	-
Ahae2126ch_RS17400	61197..62180	+	984	WP_016165175.1	sigma-54 dependent transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..70629	70629
	-	inside	IScluster/Tn	-	-	51008..60788	9780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13591.59 Da
• Isoelectric Point: 6.7180

>T110904 WP_019383563.1 NZ_CP031992:57310-57666 [Acinetobacter haemolyticus]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGHPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T110904 NZ_CP031992:57310-57666 [Acinetobacter haemolyticus]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCATCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT110904 WP_001140620.1 NZ_CP031992:57659-57961 [Acinetobacter haemolyticus]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT110904 NZ_CP031992:57659-57961 [Acinetobacter haemolyticus]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2L0I0R9