Detailed information of TA system TA110813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5492128..5492730
• Replicon: chromosome
• Accession: NZ_CP031922
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: B9S25_RS28620
• Protein ID: WP_000897305.1
• Coordinates: 5492419..5492730 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9S25_RS28615
• Protein ID: WP_000356397.1
• Coordinates: 5492128..5492418 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS28585	5487674..5488309	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
B9S25_RS28590	5488306..5489235	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
B9S25_RS28600	5489635..5490615	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
B9S25_RS28605	5490844..5491086	-	243	WP_001086390.1	hypothetical protein	-
B9S25_RS28610	5491305..5491523	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
B9S25_RS28615	5492128..5492418	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B9S25_RS28620	5492419..5492730	-	312	WP_000897305.1	hypothetical protein	Toxin
B9S25_RS28625	5492959..5493867	+	909	WP_001162704.1	alpha/beta hydrolase	-
B9S25_RS28630	5493931..5494872	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B9S25_RS28635	5494917..5495354	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B9S25_RS28640	5495351..5496223	-	873	WP_000920762.1	virulence factor BrkB family protein	-
B9S25_RS28645	5496217..5496816	-	600	WP_001315111.1	glucose-1-phosphatase	-
B9S25_RS28650	5496915..5497700	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5489635..5490615	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110813 WP_000897305.1 NZ_CP031922:c5492730-5492419 [Escherichia coli O26:H11]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110813 NZ_CP031922:c5492730-5492419 [Escherichia coli O26:H11]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110813 WP_000356397.1 NZ_CP031922:c5492418-5492128 [Escherichia coli O26:H11]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110813 NZ_CP031922:c5492418-5492128 [Escherichia coli O26:H11]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7