Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4562479..4563014 Replicon chromosome
Accession NZ_CP031922
Organism Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag B9S25_RS24130 Protein ID WP_032183258.1
Coordinates 4562727..4563014 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag B9S25_RS24125 Protein ID WP_000729704.1
Coordinates 4562479..4562739 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9S25_RS24100 4557515..4558654 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
B9S25_RS24105 4558638..4560731 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
B9S25_RS24110 4560836..4561114 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
B9S25_RS24115 4561107..4561463 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
B9S25_RS24120 4561520..4562293 - 774 WP_000543897.1 C40 family peptidase -
B9S25_RS24125 4562479..4562739 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B9S25_RS24130 4562727..4563014 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B9S25_RS24135 4563170..4563910 + 741 WP_001225679.1 murein L,D-transpeptidase -
B9S25_RS24140 4563881..4564648 - 768 WP_000333380.1 class II glutamine amidotransferase -
B9S25_RS24145 4564854..4565432 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T110809 WP_032183258.1 NZ_CP031922:4562727-4563014 [Escherichia coli O26:H11]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T110809 NZ_CP031922:4562727-4563014 [Escherichia coli O26:H11]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT110809 WP_000729704.1 NZ_CP031922:4562479-4562739 [Escherichia coli O26:H11]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT110809 NZ_CP031922:4562479-4562739 [Escherichia coli O26:H11]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References