Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2033331..2033833 Replicon chromosome
Accession NZ_CP031922
Organism Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B9S25_RS10255 Protein ID WP_000767829.1
Coordinates 2033579..2033833 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B9S25_RS10250 Protein ID WP_001259255.1
Coordinates 2033331..2033582 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9S25_RS10225 2028512..2029579 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
B9S25_RS10230 2029579..2030649 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
B9S25_RS10235 2030646..2031950 - 1305 WP_000009589.1 histidinol dehydrogenase -
B9S25_RS10240 2031956..2032855 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
B9S25_RS10245 2033001..2033048 - 48 WP_001375657.1 his operon leader peptide -
B9S25_RS10250 2033331..2033582 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B9S25_RS10255 2033579..2033833 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B9S25_RS10260 2033916..2034740 + 825 WP_000754737.1 SDR family oxidoreductase -
B9S25_RS10265 2034786..2035715 + 930 WP_000803351.1 LysR family transcriptional regulator -
B9S25_RS10270 2035930..2035992 + 63 WP_010723108.1 membrane protein YoeI -
B9S25_RS10275 2035982..2037340 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B9S25_RS10280 2037519..2038577 + 1059 WP_000492324.1 transport protein YeeE -
B9S25_RS10285 2038591..2038818 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110788 WP_000767829.1 NZ_CP031922:2033579-2033833 [Escherichia coli O26:H11]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110788 NZ_CP031922:2033579-2033833 [Escherichia coli O26:H11]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110788 WP_001259255.1 NZ_CP031922:2033331-2033582 [Escherichia coli O26:H11]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110788 NZ_CP031922:2033331-2033582 [Escherichia coli O26:H11]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References