Detailed information of TA system TA110786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1108022..1108749
• Replicon: chromosome
• Accession: NZ_CP031922
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: B9S25_RS05460
• Protein ID: WP_000547559.1
• Coordinates: 1108022..1108333 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9S25_RS05465
• Protein ID: WP_000126294.1
• Coordinates: 1108330..1108749 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS05430	1103164..1104873	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
B9S25_RS05435	1104883..1105425	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
B9S25_RS05440	1105425..1106192	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
B9S25_RS05445	1106189..1106599	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
B9S25_RS05450	1106592..1107062	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
B9S25_RS05455	1107105..1107848	+	744	WP_001341829.1	hypothetical protein	-
B9S25_RS05460	1108022..1108333	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
B9S25_RS05465	1108330..1108749	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
B9S25_RS05470	1108863..1110287	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
B9S25_RS05475	1110296..1111753	-	1458	WP_024017722.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
B9S25_RS05480	1112013..1113023	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
B9S25_RS05485	1113172..1113699	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T110786 WP_000547559.1 NZ_CP031922:1108022-1108333 [Escherichia coli O26:H11]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T110786 NZ_CP031922:1108022-1108333 [Escherichia coli O26:H11]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT110786 WP_000126294.1 NZ_CP031922:1108330-1108749 [Escherichia coli O26:H11]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT110786 NZ_CP031922:1108330-1108749 [Escherichia coli O26:H11]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163