Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2009305..2009807 Replicon chromosome
Accession NZ_CP031919
Organism Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CCU01_RS09950 Protein ID WP_000767829.1
Coordinates 2009553..2009807 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CCU01_RS09945 Protein ID WP_001259255.1
Coordinates 2009305..2009556 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU01_RS09920 2004482..2005549 - 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CCU01_RS09925 2005549..2006619 - 1071 WP_001099213.1 histidinol-phosphate transaminase -
CCU01_RS09930 2006616..2007920 - 1305 WP_001400693.1 histidinol dehydrogenase -
CCU01_RS09935 2007926..2008825 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CCU01_RS09940 2008971..2009021 - 51 WP_001364200.1 his operon leader peptide -
CCU01_RS09945 2009305..2009556 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CCU01_RS09950 2009553..2009807 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CCU01_RS09955 2009890..2010714 + 825 WP_000754755.1 SDR family oxidoreductase -
CCU01_RS09960 2010760..2011689 + 930 WP_000803351.1 LysR family transcriptional regulator -
CCU01_RS09965 2011904..2011966 + 63 WP_010723108.1 membrane protein YoeI -
CCU01_RS09970 2011956..2013314 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CCU01_RS09975 2013493..2014551 + 1059 WP_000492332.1 transport protein YeeE -
CCU01_RS09980 2014565..2014792 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110765 WP_000767829.1 NZ_CP031919:2009553-2009807 [Escherichia coli O145:NM]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110765 NZ_CP031919:2009553-2009807 [Escherichia coli O145:NM]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110765 WP_001259255.1 NZ_CP031919:2009305-2009556 [Escherichia coli O145:NM]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110765 NZ_CP031919:2009305-2009556 [Escherichia coli O145:NM]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References